Tet-On Systems For Doxycycline-inducible Gene Expression
نویسندگان
چکیده
منابع مشابه
Tet-On Systems For Doxycycline-inducible Gene Expression
The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications. These systems are based on regulatory elements that control the activity of the tetracycline-resistance operon in bacteria. The Tet-Off system allows silen...
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The ability to control transgene expression within specific tissues is an important tool for studying the molecular and cellular mechanisms of development, physiology, and disease. We developed a Tet-On system for spatial and temporal control of transgene expression in zebrafish rod photoreceptors. We generated two transgenic lines using the Xenopus rhodopsin promoter to drive the reverse tetra...
متن کاملDevelopment of single-vector Tet-on inducible systems with high sensitivity to doxycycline.
Single-vector Tet-on systems were developed to enable the tight regulation of transgenes in mammalian cells with a low dosage of doxycycline. Both the regulatory and the responsive units were integrated in a single vector and separated by a short DNA segment (214 bp). In the developed single-vector Tet-on systems, a high level of expression of the transgene can be induced by doxycycline at a co...
متن کاملOrgan-specific responses of total body irradiated doxycycline-inducible manganese superoxide dismutase Tet/Tet mice.
BACKGROUND/AIM We evaluated doxycycline-inducible manganese superoxide dismutase (MnSOD(tet/tet)) mice after 9.25 Gy total-body irradiation (TBI) or 20 Gy thoracic irradiation. MATERIALS AND METHODS Six-week-old MnSOD(tet/tet) or control C57BL/6NHsd mice on or off doxycycline (doxy) in food received 9.25 Gy TBI, were sacrificed at day 19 and bone marrow, brain, esophagus, heart, intestine, ki...
متن کاملTet B or not tet B: advances in tetracycline-inducible gene expression.
Be it the B class, or another class of tetracycline (tet) repressor, the utility and specificity of transcriptional regulators based on this family of prokaryotic DNA binding proteins is unparalleled. A method for regulating gene expression at will in mammalian cells has long been the holy grail. Transfections of uncontrolled numbers of plasmids and unregulated gene expression were breakthrough...
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ژورنال
عنوان ژورنال: Current Gene Therapy
سال: 2016
ISSN: 1566-5232
DOI: 10.2174/1566523216666160524144041